Figure 1.

RASSF5 methylation and correlation to INRG stage and MYCN amplification. (A) Examples of bisulfite sequencing of RASSF5in the RASSF5A CpG island: top sequence NB69 and bottom sequence Kelly. CpG sites are underlined and C in the sequence indicates methylation and C/T in the sequence indicate partial methylation. (B) High methylation of RASSF5 is significantly correlated to INRG stage M and MYCN amplification. P-values are indicated in the left upper corner in each graph. Box plot explanation; upper and lower hinges of the box represent the 75th percentile and 25th respectively; whiskers show highest and lowest values. Open circles represent outliers and asterisks show extremes.