Table 2.

Primers used in this study

Gene	Method	Primer sequence	Product (bp)	AT °C	Primer design
RASSF2A	MSP	F: 5′-GTTCGTCGTCGTTTTTTAGGCG-3′	109	62	[13]
		R: 5′-AAAAACCAACGACCCCCGCG-3′
RASSF2A	MSP	F: 5′-AGTTTGTTGTTGTTTTTTAGGTGG-3′	109	60	[13]
		R: 5′-AAAAAACCAACAACCCCCACA-3′
RASSF4	COBRA	F: 5′-AGGATAYGATATATGTAGTGGTTTTTGGATT-3′	270	TD 65-55	[14]
		R: 5′-ATTATAACCCCTAAATTACTTAACAAAAATACCAAA-3′
RASSF5A*	BSP	F: 5′-TTAGGAAAGAGGAATATTTTAT-3′	434	TD 60-50	[12]
		R: 5′-TAAACCTTCAACCCTACCTCTTTC-3′
RASSF5C**	BSP	F: 5′-GGGGTTTAGAGTTAGGGGTTTA-3′	345	TD 60-50	[12]
		R: 5′-TATAACTTTATCCCTTTACTA-3′
RASSF6	COBRA	F: 5′-GTATAGGGAGTGGTTTAGGTTTTTTGATAT-3′	353	TD 67-57	[10]
		R: 5′-ATCCCCATTTTTTACCTATTATTCACACTATA-3′
RASSF7	BSP	F: 5′-GAGAAAAGTTAGGTTTTAGA-3′	592	TD 62-52	[12]
		R: 5′-CTCAACAACCTTCTAATATAA-3′
RASSF8	BSP	F: 5′-TTTTATAATGTAGYGTTGGYGTTTTAGTTT-3′	374	TD 67-57	[10]
		R: 5′-CRAAACTCRACRAAACTAAACRAAAAACT-3′
RASSF10	BSP	F: 5′-TTGTTTTTGTTGTTTTYGTYGTTTTAGTAGATT-3′	634	TD 67-57	[10]
		R: 5′-CRATTAAACTTAACCAATTTACRAAAAACCTTA-3′
RASSF2A	qRT-PCR	F: 5′-AAGGGGTGGAGAGTGATATGAAGAG-3′	194	60	[15]
		R: 5′-AGGGACGTTTGGTGGCTGTAGT-3′
RASSF4	qRT-PCR	F: 5′-GGACTGCGCGATGACTGGAC-3′	126	56	[15]
		R: 5′-CCGACTTCTGAATGGACTTGCTGT-3′
RASSF5	End-point RT-PCR	F: 5′-CCTGGGCATGAAACTGAGTGAAGA-3′	188	56	Manual
		R: 5′-tgatggcatctaggggcaggtaga-3′
RASSF6	End-point RT-PCR	F: 5′-ATGGAGAGACTGAAGATGGC-3′	203	56	[15]
		R: 5′-CAGGGTGTTGCTGTGATAAG-3′
RASSF7	End-point RT-PCR	F: 5′-CAGCAGAGCGAGCCTTGCAGGCTCA-3′	149	59	Manual
		R: 5′-CTGAGTGCCAGGAGGGCCCCTGTCA-3′
RASSF10	qRT-PCR	F: 5′-CCATGACCCAGGAGAAACAG-3′	226	60	[10]
		R: 5′-TGCTGGCGAATTGTGTGGTC-3′

*cg17558126, **cg02589695.

MSP, Methylation specific PCR; BSP, Bisulfite sequencing PCR; COBRA, Combined bisulfite restriction PCR.

qRT-PCR, Quantitative real time PCR; AT, Annealing temperature; TD, Touch down.