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Vascular island proliferation during microvascular network growth. A-I) BRDU-positive cells (arrowheads) along PECAM labeled vascular islands (arrows) were observed on day 3 post compound 48/80 stimulation. Vascular islands in some cases contained multiple proliferating cells and endothelial cell branches. J-L) BRDU co-localized with DAPI-positive nuclei along the disconnected PECAM-positive endothelial cell segments. Scale bars = 20 μm (A-F, J-L), 50 μm (G-I).
