Figure 1.

Schematic diagram of one-pot bioethanol production. SF was used as an inducing substrate for cellulase production by A. cellulolyticus C-1 at 28°C. (A) A. cellulolyticus cells produce cellulase from SF, and subsequently the produced cellulase saccharifies SF. S. cerevisiae cells consume the liberated glucose from the saccharification of SF and produce ethanol. The overall process from cellulase production to ethanol generation is carried out in a single reactor. (B) The first step of the one-pot bioethanol production is cellulase production by A. cellulolyticus cells and the second step is simultaneous saccharification of SF and ethanol production by the addition of S. cerevisiae inoculum and SF