Table 3.
Transmission of p5cr mutant alleles by self-pollination and in reciprocal crosses with wildtype plants
| Genotype pollen donor1 | Genotype pollen recipient1 | Offspring with p5cr allele [% ±SD2] | Expected frequency [%] | Seedlings analysed |
|---|---|---|---|---|
| D/d1 |
D/d1 |
68.5 ±3.6 |
66.7 |
773 |
| D/d2 |
D/d2 |
68.4 ±1.5 |
66.7 |
884 |
| D/D |
D/d1 |
50.0 |
50 |
702 |
| D/D |
D/d2 |
45.8* |
50 |
783 |
| D/d1 |
D/D |
48.5 |
50 |
581 |
| D/d2 | D/D | 46.1 | 50 | 596 |
Transmission of the p5cr mutant alleles was determined by kanamycin resistance of the progeny after selfing (first two rows) or crossing of various parental genotypes.
1 D: P5CR wildtype allele; d1: p5cr-1 mutant allele; d2: p5cr-2 mutant allele.
2 SD was calculated from five batches of seeds from individual parent plants.
* indicates significant difference from the expected value (p<0.05 by Χ2 test with 1 degree of freedom).