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FCV p30 is ER localized and does not induce Golgi disruption. CRFK cells were transfected to express GFP or GFP-tagged PV 3A, NV p22 or FCV p30 and immuno-stained (Alexa-Fluor 594 secondary antibody, red fluorescence) for the cis Golgi marker protein GM130 (A) or the ER marker protein disulfide isomerase (PDI) (B). Nuclei were counterstained with DAPI (blue fluorescence) and cells were imaged by deconvolution microscopy.
