Table 2.
qPCR data of Lactobacillus, Bifidobacterium, Atopobium and Prevotella
| ng of target DNA/μg vaginal genomic DNA (mean ± SD) | |||||
|---|---|---|---|---|---|
|
Woman N. |
Time point |
Lactobacillus |
Bifidobacterium |
Atopobium |
Prevotella |
| Probiotic (P) |
|
|
|
|
|
| 1 |
W33 |
2.4 × 101 ± 1.1 |
1.9 × 10-2 ± 7.4 × 10-3 |
3.6 ± 1.5 |
2.1 × 10-2 ± 1.0 × 10-2 |
| |
W37 |
3.0 × 101 ± 3.1 |
3.1 × 10-2 ± 2.7 × 10-4 |
1.3 × 101 ± 6.8 |
9.1 × 10-2 ± 1.6 × 10-2 |
| 2 |
W33 |
9.6 ± 8.7 × 10-1 |
3.1 × 10-2 ± 8.8 × 10-3 |
5.4 × 101 ± 7.4 |
1.4 × 10-1 ± 4.8 × 10-2 |
| |
W37 |
5.9 × 10-1 ± 4.9 × 10-2 |
2.4 × 10-2 ± 1.2 × 10-2 |
2.4 × 101 ± 1.9 × 101 |
1.1 × 10-1 ± 1.1 × 10-2 |
| 3 |
W33 |
2.4 × 101 ± 2.9 |
2.4 × 10-2 ± 4.2 × 10-3 |
1.1 × 101 ± 6.0 |
1.1 × 10-1 ± 7.7 × 10-3 |
| |
W37 |
2.2 × 101 ± 2.4 |
3.0 × 10-2 ± 2.4 × 10-3 |
4.0 ± 2.3 |
5.2 × 10-2 ± 8.2 × 10-3 |
| 4 |
W33 |
2.2 × 101 ± 2.0 |
6.8 × 10-2 ± 8.3 × 10-3 |
4.7 ± 1.9 |
7.3 × 10-2 ± 2.9 × 10-2 |
| |
W37 |
1.5 × 101 ± 1.4 |
2.1 × 10-2 ± 7.2 × 10-3 |
5.2 ± 2.0 |
4.6 × 10-2 ± 9.5 × 10-3 |
| 5 |
W33 |
2.5 × 101 ± 4.5 |
2.1 × 10-2 ± 3.4 × 10-3 |
1.2 × 101 ± 3.0 |
9.3 × 10-2 ± 8.3 × 10-3 |
| |
W37 |
2.2 x 101 ± 4.5 |
1.4 x 10-2 ± 3.2 x 10-3 |
1.5 x 101 ± 1.9 |
3.0 x 10-2 ± 1.1 x 10-2 |
| 6 |
W33 |
1.1 × 10-1 ± 3.4 × 10-3 |
7.1 × 10-2 ± 7.1 × 10-3 |
1.0 × 101 ± 4.1 |
1.2 × 10-1 ± 1.3 × 10-2 |
| |
W37 |
2.2 ± 6.0 × 10-1 |
2.1 ± 1.7 × 10-1 |
2.4 × 101 ± 1.0 × 101 |
1.5 × 10-1 ± 1.2 × 10-2 |
| 7 |
W33 |
4.1 × 101 ± 8.5 |
3.7 × 10-2 ± 5.4 × 10-3 |
2.9 × 101 ± 9.2 |
1.2 × 10-1 ± 2.1 × 10-2 |
| |
W37 |
2.0 × 101 ± 2.6 |
1.7 × 10-2 ± 4.4 × 10-3 |
2.6 × 101 ± 7.7 |
1.1 × 10-1 ± 1.1 × 10-3 |
| 8 |
W33 |
1.0 × 101 ± 1.7 × 10-1 |
1.3 × 10-2 ± 1.9 × 10-3 |
5.5 ± 1.2 |
4.2 × 10-2 ± 1.9 × 10-2 |
| |
W37 |
2.1 × 101 ± 2.0 |
1.5 × 10-2 ± 2.6 × 10-3 |
1.6 × 101 ± 6.6 |
5.1 × 10-2 ± 3.3 × 10-3 |
| 9 |
W33 |
0.0 ± 0.0 |
7.1 × 10-3 ± 2.8 × 10-5 |
1.8 × 101 ± 7.1 |
6.7 × 10-2 ± 1.5 × 10-2 |
| |
W37 |
0.0 ± 0.0 |
1.1 × 101 ± 1.0 |
1.5 × 101 ± 6.8 |
2.3 × 10-1 ± 8.0 × 10-2 |
| 10 |
W33 |
6.7 ± 6.1 × 10-1 |
2.0 × 10-2 ± 4.8 × 10-3 |
1.4 × 101 ± 4.3 |
8.6 × 10-2 ± 2.0 × 10-2 |
| |
W37 |
1.1 × 101 ± 1.4 |
2.3 × 10-2 ± 1.5 × 10-2 |
1.7 × 101 ± 9.7 |
8.0 × 10-2 ± 2.9 × 10-2 |
| 11 |
W33 |
2.7 × 101 ± 1.7 |
2.9 x 10-3 ± 1.7 × 10-3 |
2.3 ± 1.8 |
3.2 × 10-2 ± 3.3 × 10-3 |
| |
W37 |
3.0 × 101 ± 5.6 |
1.3 x 10-2 ± 8.5 × 10-3 |
1.3 ± 7.5 × 10-1 |
3.6 × 10-2 ± 1.3 × 10-2 |
| 12 |
W33 |
2.2 ± 5.6 × 10-1 |
1.5 × 101 ± 2.3 |
1.4 × 101 ± 2.9 |
2.2 × 10-1 ± 2.1 × 10-2 |
| |
W37 |
2.0 ± 3.1 × 10-1 |
8.7 ± 5.6 × 10-1 |
1.2 × 101 ± 2.3 |
1.0 × 10-1 ± 1.8 × 10-2 |
| 13 |
W33 |
3.7 × 101 ± 5.4 |
3.0 × 10-2 ± 4.5 × 10-3 |
7.0 ± 2.6 × 10-1 |
2.7 × 10-2 ± 5.0 × 10-4 |
| |
W37 |
6.6 × 101 ± 5.9 |
1.1 × 10-2 ± 2.2 × 10-3 |
6.8 ± 6.6 × 10-1 |
5.7 × 10-2 ± 2.0 × 10-3 |
| 14 |
W33 |
2.2 × 101 ± 8.5 |
1.7 × 10-2 ± 4.9 × 10-3 |
9.0 ± 4.4 × 10-1 |
6.7 × 10-2 ± 6.6 × 10-3 |
| |
W37 |
1.6 × 101 ± 4.9 |
2.8 × 10-2 ± 4.7 × 10-3 |
1.1 × 101 ± 1.1 |
1.1 × 10-1 ± 1.8 × 10-3 |
| 15 |
W33 |
2.2 × 101 ± 7.1 |
1.4 × 10-2 ± 7.1 × 10-3 |
1.8 × 101 ± 5.6 |
1.1 × 10-1 ± 1.4 × 10-2 |
| |
W37 |
2.8 × 101 ± 3.4 |
4.7 × 10-3 ± 2.3 × 10-3 |
1.1 × 101 ± 2.4 × 10-1 |
7.4 × 10-2 ± 2.4 × 10-3 |
| Control (C) |
|
|
|
|
|
| 16 |
W33 |
5.4 × 101 ± 4.0 |
2.1 × 10-2 ± 5.6 × 10-3 |
1.1 × 101 ± 4.6 |
6.8 × 10-2 ± 1.1 × 10-2 |
| |
W37 |
2.0 × 101 ± 1.7 |
2.0 × 10-2 ± 7.4 × 10-3 |
1.4 × 101 ± 5.0 |
5.6 × 10-2 ± 5.4 × 10-3 |
| 17 |
W33 |
5.5 ± 5.3 × 10-1 |
6.0 ± 1.6 × 10-1 |
1.2 × 101 ± 4.3 |
5.9 × 10-2 ± 2.3 × 10-2 |
| |
W37 |
1.5 × 101 ± 2.9 |
9.3 ± 5.3 × 10-1 |
1.9 × 101 ± 8.7 |
5.4 × 10-2 ± 1.0 × 10-2 |
| 18 |
W33 |
2.6 ± 1.6 × 10-1 |
1.8 ± 3.5 × 10-2 |
1.3 × 101 ± 5.5 |
8.8 × 10-2 ± 1.7 × 10-2 |
| |
W37 |
1.2 × 101 ± 2.0 |
2.9 ± 7.5 × 10-2 |
3.3 × 101 ± 4.4 |
4.5 × 10-2 ± 2.8 × 10-3 |
| 19 |
W33 |
7.6 × 101 ± 3.3 × 10-1 |
1.2 ± 7.9 × 10-3 |
1.3 × 101 ± 3.6 |
1.9 × 10-1 ± 3.2 × 10-3 |
| |
W37 |
2.7 × 101 ± 3.8 |
2.7 × 10-2 ± 4.7 × 10-3 |
8.2 ± 4.6 |
1.1 × 10-1 ± 2.6 × 10-2 |
| 20 |
W33 |
1.6 × 101 ± 1.4 |
1.1 × 101 ± 1.2 |
1.2 × 101 ± 5.5 |
8.6 × 10-2 ± 1.5 × 10-2 |
| |
W37 |
1.0 × 101 ± 6.4 × 10-2 |
1.1 × 101 ± 1.4 |
1.2 × 101 ± 4.7 |
1.1 × 10-1 ± 3.1 × 10-2 |
| 21 |
W33 |
5.6 × 101 ± 8.3 |
1.7 × 10-2 ± 1.7 × 10-3 |
2.1 × 101 ± 1.0 × 101 |
1.3 × 10-1 ± 2.0 × 10-2 |
| |
W37 |
6.4 × 101 ± 1.5 |
3.3 × 10-2 ± 8.7 × 10-3 |
2.2 × 101 ± 1.0 × 101 |
1.2 × 10-1 ± 2.4 × 10-2 |
| 22 |
W33 |
4.3 × 101 ± 2.0 |
1.2 × 10-1 ± 2.8 × 10-2 |
2.3 × 10-1 ± 1.5 × 10-2 |
0.0 ± 0.0 |
| |
W37 |
6.8 × 101 ± 5.1 |
2.7 × 10-2 ± 6.6 × 10-3 |
1.9 × 10-1 ± 2.0 × 10-2 |
0.0 ± 0.0 |
| 23 |
W33 |
2.6 × 101 ± 5.6 |
2.3 × 10-1 ± 3.6 × 10-2 |
0.0 ± 0.0 |
0.0 ± 0.0 |
| |
W37 |
6.3 × 101 ± 2.0 |
8.2 × 10-3 ± 1.9 × 10-3 |
1.6 × 10-1 ± 2.9 × 10-2 |
5.3 × 10-1 ± 1.8 × 10-1 |
| 24 |
W33 |
1.2 × 101 ± 1.0 |
2.7 × 101 ± 2.1 × 10-1 |
1.8 ± 1.5 × 10-1 |
6.8 × 10-1 ± 3.4 × 10-2 |
| |
W37 |
7.5 × 101 ± 3.8 |
9.7 × 10-3 ± 3.7 × 10-3 |
3.7 × 10-1 ± 3.4 × 10-2 |
0.0 ± 0.0 |
| 25 |
W33 |
6.5 × 101 ± 1.0 × 101 |
3.0 × 10-2 ± 1.0 × 10-2 |
7.5 × 10-2 ± 7.5 × 10-3 |
0.0 ± 0.0 |
| |
W37 |
6.6 × 101 ± 7.1 |
9.1 × 10-3 ± 5.1 × 10-4 |
2.5 × 10-1 ± 2.7 × 10-2 |
0.0 ± 0.0 |
| 26 |
W33 |
8.5 × 101 ± 6.3 |
4.4 ± 9.3 × 10-1 |
3.2 × 10-1 ± 3.9 × 10-2 |
0.0 ± 0.0 |
| |
W37 |
5.4 × 101 ± 4.5 |
2.0 × 10-2 ± 6.1 × 10-4 |
3.6 × 10-1 ± 4.2 × 10-2 |
0.0 ± 0.0 |
| 27 |
W33 |
7.0 × 101 ± 1.5 × 101 |
3.3 × 10-2 ± 4.7 × 10-3 |
2.8 × 10-1 ± 2.6 × 10-2 |
0.0 ± 0.0 |
| W37 | 6.6 × 101 ± 3.6 × 10-1 | 2.1 × 10-2 ± 1.6 × 10-2 | 4.0 × 10-1 ± 3.8 × 10-2 | 0.0 ± 0.0 | |