Figure 7.

In vitro cytokine response of splenocytes from LPS-treated and control LPS-untreated B10.BR males to LPS stimulation. TNFα, IL-6, IFNγ, TGFβ and IL-10 in cell supernatants were determined. No significant differences were found between LPS-treated males and the control group after the second LPS dose (A). After the fourth LPS dose the IL-6 level was significantly increased in LPS-treated males (B). At the end of the experiment splenocytes from LPS-treated males responded to LPS stimulation by significantly higher production of TNFα and IL-6 cytokines (C). * p < 0.05, ** p < 0.01.