Table 1. Alternative splicing events in RVE8.
| Primer pair | AS event# | RT-PCR size (bp) |
Description | Outcome |
|---|---|---|---|---|
| |
|
|
|
|
|
RVE8#1 |
FS |
371 |
Fully spliced |
|
| |
AS1 |
440 |
I3R |
PTC |
| |
AS2 |
658 |
I1R |
PTC |
| |
AS3 |
727 |
I1R and I3R |
PTC |
| |
|
|
|
|
| |
|
|
|
|
|
RVE8#2 |
FS |
474 |
Fully spliced |
|
| |
AS4 |
457 |
Alternative 3′ss in exon 5 (removes 17 nt) |
PTC; downstream splice junctions; likely NMD |
| |
AS5 |
479 |
Alternative 3′ss in exon 5 (removes 17 nt) plus alternative 3′ss in intron 7 (adds 22 nt) |
PTC; downstream splice junctions; likely NMD |
| |
AS6 |
496 |
Alternative 3′ss in intron 7 (adds 22 nt) (TAIR.2) |
PTC; downstream splice junctions; likely NMD |
| |
AS7 |
592 |
I4R |
PTC |
| |
AS8 |
614 |
I4R plus alt 3′ss in intron 7 adds 22nt |
PTC; downstream splice junctions; likely NMD |
| |
AS9 |
668 |
I7R |
PTC |
| |
AS10 |
786 |
I4R+I7R |
PTC |
FS, fully spliced; AS, alternatively spliced; ss, splice site; INR, intron retention of intron “N”; PTC, premature termination codon; NMD, nonsense-mediated decay.