Figure 1. Overexpression of FUS3 or AKIN10 leads to ABA hypersensitivity and delayed germination, which is partly dependent on de novo ABA synthesis. Germination (radicle protrusion) kinetics of seeds from WT and two independent lines of ML1:FUS3 (A) or 35S:AKIN10 (B) on MS media or MS supplemented with 0.2 μM ABA or 10 µM fluridone (FLU). WT germination is significantly higher (p < 0.01) than 35S:AKIN10 on 10 µM FLU at 2 d after imbibition (DAI). (C) ABA dose-response curves for WT, ML1:FUS3 and 35S:AKIN10 seed germination (radicle protrusion) 2 d after imbibition. WT germination is higher (p < 0.001) than ML1:FUS3 and 35S:AKIN10 at 0.4 µM ABA. Averages from 3 experiments ± SD are shown. 100–150 seeds were used in each experiment.