Figure 2. Impact of GSNO, DEANO and GSH on the concentrations of iron, manganese, zinc and copper in roots of plants exposed to iron deficiency. (A) Iron concentration, (B) copper concentration, (C) zinc concentration, (D) manganese concentration. Plants were cultivated 4 weeks in iron-sufficient conditions (Fe 50 µM) and then transferred 48 h in iron deficient (Fe 5 µM) conditions. Then, plants were treated with GSNO (50 µM), GSH (50 µM) or DEANO (50 µM) for 48 h. The concentration of metals was determined in the roots by ICP-OES. Each value represents the means ± SD of nine measurements (three replicates per experiment performed three times). Stars represent significant differences from control according to ANOVA on ranks followed by a comparison vs. control (p < 0.05).