Figure 5. Oridonin modulated the expression of pro-apoptotic protein Bax and anti-apoptotic protein Bcl-2 in SUP-B15 cells. The cells were incubated with 7.5 μM oridonin for 6, 12, 24 h (A), or with 5, 10, 15 μM doses of oridonin for 24 h (B). Equal amounts of protein from each sample were separated on SDS-PAGE and immunoblotted with antibodies against Bcl2 and Bax. GAPDH was used as a loading control. The results shown were representative of two independent experiments. Oridonin upregulated the expression of pro-apoptotic protein Bax and downregulated the expression of anti-apoptotic protein Bcl-2 in SUP-B15 cells.