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. 2012 Nov 8;8(11):e1003019. doi: 10.1371/journal.pgen.1003019

Figure 4. S. mediterranea foxJ1-4 is required for the differentiation of motile cilia.

Figure 4

(A–C) Control (A), ift172(RNAi) (B) and foxJ1-4(RNAi) worms (C) 14 days after the last RNAi feeding. Worms shown in panels B and C display tissue edema. Scale bar: 1 mm. (D–F) Anti-α-tubulin staining (green) of the multi-ciliated ventral epithelium in control (D), ift172(RNAi) (E) and foxJ1-4(RNAi) worms (F), respectively. The even spacing of nuclei (magenta) characteristic of the ventral epithelium demonstrates epithelial integrity in E and F. Images are single optical sections. Scale bar: 20 µm. (G) foxJ1-4 expression is unaffected by control RNAi (dsred). (H) foxJ1-1 expression is not altered in a foxJ1-4(RNAi) worm. (I) foxJ1-4 expression is substantially reduced in a foxJ1-4(RNAi) worm. Scale bar: 200 µm.