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IGF1R juxtamembrane domain analogs selectively inhibited IGF-1, but not insulin-induced AKT activation in breast cancer cells. AKT activity in cell lysates was determined with the help of the Omni fluorogenic peptide substrate after 20 min incubation with IGF-1 (25 ng/ml) or insulin. A non-optimized analogue of insulin receptor juxtamembrane domain IR-2 (Pal-RQPDGPLGPLY-NH2) was used as a positive control in inhibition of insulin-induced AKT activation.
