Table 1. GIA EC50 values of anti-PfRH5 and anti-PfAMA1 IgG against various Cambodian parasite isolates and the 3D7 parasite clone.
| Parasite | EC50 (mg/mL total IgG, anti-PfAMA1 IgG) | EC50 (mg/mL total IgG, anti-PfRH5 IgG) | EC50 (µg/mL PfAMA1-specific IgG) | EC50 (µg/mL PfRH5-specific IgG) |
| CP803 | 6.2 | 0.3 | 151 | 12 |
| CP806 | 7.8 | 1.5 | 167 | 68 |
| CP830 | 5.3 | 0.8 | 152 | 30 |
| CP845 | 5.5 | 0.2 | 143 | 9 |
| CP887 | 7.9 | 0.7 | 227 | 22 |
| 3D7 | 3.1 (2.27–3.81) | 1.5 (0.95–2.01) | 113 | 50 |
Total IgG EC50 value for Cambodian parasite isolates is the median of observed values for five individual rabbits; values in italics are extrapolated from observed values. Antigen-specific antibody EC50 values are derived from fitting of a single curve to all available GIA data points for each antigen and each parasite (as depicted in Figure 1E and Figure 2). For comparison, estimated EC50 values of the total and antigen-specific IgG against the 3D7 parasite clone are also shown. 3D7 antigen-specific IgG values are as calculated in Figure 1E, whereas total IgG values are a mean, with 95% CI in parentheses, from eight independent experiments that have estimated the total IgG EC50 of anti-PfRH5 and anti-PfAMA1, with a total of five immunized rabbits for each antigen (Figure 1B, Figure 4A–B, Reference 17]).