Figure 9. PF-46396 can rescue the assembly/release defect of some but not all MHR mutants.

Top panel: Radioimmunoprecipitation analysis of cell- and virion-associated proteins in the absence and presence of 5 µM PF-46396. Positions of Pr55^Gag (PrGag), Pr41^Gag (p41), CA-SP1, and CA are indicated. Lower panel shows phosphorimager-based quantification of relative virus release efficiency (VRE), calculated as described in the Fig. 6 legend. Error bars denote SD; N = 3. Note that PF-46396 significantly enhances the release of virus particles for E159Q (and G156E, as shown earlier) but not for Q155N, G156V, or E159D.