Figure 3. Effect of supplementation with powdered persimmon leaf on hepatic oxidative stress in C57BL/KsJ-db/db mice.

A; hepatic TBARS level. B; hepatic mitochondria hydrogen peroxide content. C; hepatic antioxidant enzyme activity. D; hepatic antioxidant gene expression. The mRNA levels were analyzed by Northern blot analysis using SOD, CAT and GPX probes and normalized to an internal control (GAPDH). Three independent analyses were performed. Values are the mean ± S.E., n = 10. *p<0.05, **p<0.01, ***p<0.001. CAT; catalase, GPX; glutathione peroxidase, GAPDH; glyceraldehyde-3-phosphate dehydrogenase, PL; powdered persimmon leaf, SOD; superoxide dismutase.