Figure 3. Average proportion of reads classified at different ranks in four environmental datasets.

The CREST LCAClassifier (analogous to MEGAN) was tested using the full SilvaMod and Greengenes [21] reference databases with their respective taxonomies, as well as the RDP Classifier [22] retrained with Greengenes (99%OTU dataset; executed via QIIME) and version 6 of the default RDP training dataset.