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. 2012 Nov 8;7(11):e49462. doi: 10.1371/journal.pone.0049462

Figure 4. Restoration of MEG3-RNA and DLK1 mRNA expression after DNMT1 knock down and reduction of methylation at MEG3 imprinting loci.

Figure 4

(A) Quantitative MEG3 RNA and (B) DLK1 mRNA expression levels in HLE cells after DNMT1 knockdown after normalization to GUSB and GAPDH levels (p = 0.029). (C) Reduction of DNA methylation at DLK1/MEG3 imprinting locus after DNMT1 knock down (DLK1p: DLK1 promoter). Methylation analysis was performed using quantitative pyrosequencing. Paired t-test showed significant decrease of methylation after DNMT1 knockdown for all six regions. Values shown for qRT-PCR and DNA methylation analysis are means from two independent DNMT1-knockdown experiments (50 nM and 100 nM) and two different negative control siRNAs. * = 0.001<p<0.05, *** = p<0.0001.