Rat primer: |
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TGFβ |
5′-TGG AAG TGG ATC CAC GCG CCC AAG G-3′
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5′-GCA GGA GCG CAC GAT CAT GTT GGA C-3′
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CTGF |
5′-TGT GCA CTG CCA AAG ATG GT-3′
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5′-GGT ACA CGG ACC CAC CGA-3′
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LTBP-1 |
5′-CGG ATC CCC CTA TGA TCT CA-3′
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5′-TGA CGA GGC GGT AGC AGG-3′
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Collagen I |
5′-TCA CCT ACA GCA CGC TTG-3′
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5′-GGT CTG TTT CCA GGG TTG-3′
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α8 integrin |
5′-TCC AAA TCA GAA GCT CCA ACA A-3′
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5′-CGC TCA CGA AAT TGC TGT CA-3′
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β1 integrin |
5′-AAG TCC CAA GTG CCA TGA GG-3′
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5′-CTG CAG GCT CCA CAC TCA AAT-3′
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Fibrillin-1 |
5′-TGC TCT GAA AGG ACC CAA TGT-3′
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5′-CGG GAC AAC AGT ATG CGT TAT AAC-3′
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Fibronectin |
5′-TTG CAA CCC ACC GTG GAG TAT GTG-3′
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5′-CTC GGT AGC CAG TGA GCT TAA CAC-3′
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Osteopontin |
5′-AAA GTG GCT GAG TTT GGC AG-3′
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5′-AAG TGG CTA CAG CAT CTG AGT GT-3′
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probe 5′ (FAM)-TCA GAG GAG AAG GCG CAT TAC AGC A-(TAMRA)3′ |
Vitronectin |
5′-GCT GAC CAA GAG TCA TGC AA-3′
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5′-GGT TTC CTC CGG GTA GTC AT-3′
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Nephronectin |
5′-AGC CAA CAA CAA GAC CTA CAC-3′
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5′-GCC GTG GAA TGA ACA CAA TCT C-3′
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18S |
5′-TTG ATT AAG TCC CTG CCC TTT GT-3′
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5′-CGA TCC GAG GGC CTC ACT A-3′
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Mouse primer: |
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α8 integrin |
5′-AGA ATG ATT ACC CAG ATT TGC TTG T-3′
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5′-GCT ACT TTC CCT TTT CCA AAT GC-3′
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Smooth muscle actin |
5′-CCC TGA AGA GCA TCC GAC AC-3′
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5′-GCC TTA GGG TTC AGT GGT GC-3′
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Collagen I |
5′-TCA CCT ACA GCA CCC TTG TGG-3′
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5′-CCC AAG TTC CGG TGT GAC TC-3′
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Fibronectin |
5′-TGT GAC CAG CAA CAC GGT G-3′
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5′-ACA ACA GGA GAG TAG GGC GC-3′
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Osteopontin |
5′-TTT GCT TTT GCC TGT TTG GC-3′
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5′-CAG TCA CTT TCA CCG GGA GG-3′
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Fibrillin-1 |
5′-ACA GGT CAA TGC AAC GAT CG-3′
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5′-GCA TAT GTT CGG GAT TTC TTG G-3′
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