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. 2012 Nov 8;7(11):e48362. doi: 10.1371/journal.pone.0048362

Table 1. Primer pairs for Sybr green and QT PCR analysis.

forward reverse
Rat primer:
TGFβ 5′-TGG AAG TGG ATC CAC GCG CCC AAG G-3′ 5′-GCA GGA GCG CAC GAT CAT GTT GGA C-3′
CTGF 5′-TGT GCA CTG CCA AAG ATG GT-3′ 5′-GGT ACA CGG ACC CAC CGA-3′
LTBP-1 5′-CGG ATC CCC CTA TGA TCT CA-3′ 5′-TGA CGA GGC GGT AGC AGG-3′
Collagen I 5′-TCA CCT ACA GCA CGC TTG-3′ 5′-GGT CTG TTT CCA GGG TTG-3′
α8 integrin 5′-TCC AAA TCA GAA GCT CCA ACA A-3′ 5′-CGC TCA CGA AAT TGC TGT CA-3′
β1 integrin 5′-AAG TCC CAA GTG CCA TGA GG-3′ 5′-CTG CAG GCT CCA CAC TCA AAT-3′
Fibrillin-1 5′-TGC TCT GAA AGG ACC CAA TGT-3′ 5′-CGG GAC AAC AGT ATG CGT TAT AAC-3′
Fibronectin 5′-TTG CAA CCC ACC GTG GAG TAT GTG-3′ 5′-CTC GGT AGC CAG TGA GCT TAA CAC-3′
Osteopontin 5′-AAA GTG GCT GAG TTT GGC AG-3′ 5′-AAG TGG CTA CAG CAT CTG AGT GT-3′
probe 5′ (FAM)-TCA GAG GAG AAG GCG CAT TAC AGC A-(TAMRA)3′
Vitronectin 5′-GCT GAC CAA GAG TCA TGC AA-3′ 5′-GGT TTC CTC CGG GTA GTC AT-3′
Nephronectin 5′-AGC CAA CAA CAA GAC CTA CAC-3′ 5′-GCC GTG GAA TGA ACA CAA TCT C-3′
18S 5′-TTG ATT AAG TCC CTG CCC TTT GT-3′ 5′-CGA TCC GAG GGC CTC ACT A-3′
Mouse primer:
α8 integrin 5′-AGA ATG ATT ACC CAG ATT TGC TTG T-3′ 5′-GCT ACT TTC CCT TTT CCA AAT GC-3′
Smooth muscle actin 5′-CCC TGA AGA GCA TCC GAC AC-3′ 5′-GCC TTA GGG TTC AGT GGT GC-3′
Collagen I 5′-TCA CCT ACA GCA CCC TTG TGG-3′ 5′-CCC AAG TTC CGG TGT GAC TC-3′
Fibronectin 5′-TGT GAC CAG CAA CAC GGT G-3′ 5′-ACA ACA GGA GAG TAG GGC GC-3′
Osteopontin 5′-TTT GCT TTT GCC TGT TTG GC-3′ 5′-CAG TCA CTT TCA CCG GGA GG-3′
Fibrillin-1 5′-ACA GGT CAA TGC AAC GAT CG-3′ 5′-GCA TAT GTT CGG GAT TTC TTG G-3′