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. 2012 Nov 8;7(11):e47366. doi: 10.1371/journal.pone.0047366

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© 2012 Roeseler et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Schematic representation of the HA-GBX2 and HA-GBX2ΔHD recombinant proteins containing the proline-rich region (PR), DNA-binding homeodomain (HD), and the HA epitope tag located at the amino terminus. Immunoflouresence of transiently transfected human PC-3 cells with HA-GBX2 (C, D), and, HA-Gbx2ΔHD (F, G). Blue channel identifies DAPI staining in the nucleus (B, E). Green channel identifies GFP-GBX2 fusion proteins. (D, G) Merge displays nuclear localization of GFP-GBX2 fusion proteins. Western blots of total lysates (H) and HA-immunoprecipitated samples (I) from mock, HA-Gbx2, and HA-Gbx2ΔHD transfected PC-3 cells.