Figure 3. Effects of DA extract on β-HB-induced TGF-β receptor expression in NRK cells.

(A): NRK Cells were treated with β-HB (10 mM) in 5% FCS for 48 h, followed by treatment with DA water extract of for another 24 h. For Western blot analysis (A), cell extracts were subjected to SDS-PAGE and immunoblotted with primary antibodies against TβRI and TβRII. β-actin was used as a internal control. The expression of TβRI (B) and TβRII (C) proteins was normalized to that of β-actin. Results are expressed as means ±SD of three observations. DA extract significantly reduced the β-HB-induced increases in the TβRII expression level. ^# P<0.05, *P<0.05, **P<0.01.