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. 1981 Dec;78(12):7801–7805. doi: 10.1073/pnas.78.12.7801

Calcitonin: regional distribution of the hormone and its binding sites in the human brain and pituitary.

J A Fischer, P H Tobler, M Kaufmann, W Born, H Henke, P E Cooper, S M Sagar, J B Martin
PMCID: PMC349359  PMID: 6950419

Abstract

Immunoreactive calcitonin (CT), indistinguishable from human CT-(1-32) and its sulfoxide, has been identified in extracts of the hypothalamus, the pituitary, and the thyroid obtained from human subjects at autopsy. DCT concentrations were highest in a region encompassing the posterior hypothalamus, the median eminence, and the pituitary; intermediate in the substantia nigra, the anterior hypothalamus, the globus pallidus, and the inferior colliculus; and low in the caudate nucleus, the hippocampus, the amygdala, and the cerebral and cerebellar cortices. Specific CT binding measured with 125I-labeled salmon CT was highest in homogenates of the posterior hypothalamus and the median eminence, shown to contain the highest concentrations of endogenous CT in the brain; CT binding was less than 12% of hypothalamic binding in all of the other regions of the brain examined and was negligible in the pituitary. Half-maximal binding was achieved with 0.1 nM nonradioactive salmon CT-(1-32), and the binding was directed to structural or conformational sites, or both, in the COOH-terminal half of salmon CT. The rank order of the inhibition of the binding by CT from different species and analogues of the human hormone was the same as in receptors on a human lymphoid cell line (Moran, J., Hunziker, W. & Fischer, J. A. (1978) Proc. Natl. Acad. Sci. USA 75, 3984-3988). The functional role of CT and of its binding sites in the brain remains to be elucidated.

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Selected References

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  1. Becker K. L., Geelhoed G., O'Neill W., Monaghan K. G., Snider R. H., Moore C. F., Silva O. L. Calcitonin in tissues of thyroidectomized monkey. Experientia. 1980 May 15;36(5):609–610. doi: 10.1007/BF01965831. [DOI] [PubMed] [Google Scholar]
  2. Becker K. L., Silva O. L., Post R. M., Ballenger J. C., Carman J. S., Snider R. H., Moore C. F. Immunoreactive calcitonin in cerebrospinal fluid of man. Brain Res. 1980 Aug 4;194(2):598–602. doi: 10.1016/0006-8993(80)91249-4. [DOI] [PubMed] [Google Scholar]
  3. Becker K. L., Snider R. H., Moore C. F., Monoghan K. G., Silva O. L. Calcitonin in extrathyroidal tissues of man. Acta Endocrinol (Copenh) 1979 Dec;92(4):746–751. doi: 10.1530/acta.0.0920746. [DOI] [PubMed] [Google Scholar]
  4. Bergland R., Blume H., Hamilton A., Monica P., Paterson R. Adrenocorticotropic hormone may be transported directly from the pituitary to the brain. Science. 1980 Oct 31;210(4469):541–543. doi: 10.1126/science.6252607. [DOI] [PubMed] [Google Scholar]
  5. Braga P., Ferri S., Santagostino A., Olgiati V. R., Pecile A. Lack of opiate receptor involvement in centrally induced calcitonin analgesia. Life Sci. 1978 Mar;22(11):971–977. doi: 10.1016/0024-3205(78)90362-4. [DOI] [PubMed] [Google Scholar]
  6. Catherwood B. D., Deftos L. J. Presence by radioimmunoassay of a calcitonin-like substance in porcine pituitary glands. Endocrinology. 1980 Jun;106(6):1886–1891. doi: 10.1210/endo-106-6-1886. [DOI] [PubMed] [Google Scholar]
  7. Catherwood B. D., Deftos L. J. Reactivity of ACTH and synthetic ACTH peptides with antisera to human calcitonin. J Immunol Methods. 1979;31(3-4):315–322. doi: 10.1016/0022-1759(79)90144-3. [DOI] [PubMed] [Google Scholar]
  8. Cooper C. W., Peng T. C., Obie J. F., Garner S. C. Calcitonin-like immunoreactivity in rat and human pituitary glands: histochemical, in vitro, and in vivo studies. Endocrinology. 1980 Jul;107(1):98–107. doi: 10.1210/endo-107-1-98. [DOI] [PubMed] [Google Scholar]
  9. Cooper P. E., Fernstrom M. H., Rorstad O. P., Leeman S. E., Martin J. B. The regional distribution of somatostatin, substance P and neurotensin in human brain. Brain Res. 1981 Aug 10;218(1-2):219–232. doi: 10.1016/0006-8993(81)91302-0. [DOI] [PubMed] [Google Scholar]
  10. Deftos L. J., Burton D., Catherwood B. D., Bone H. G., Parthemore J. G., Guillemin R., Watkins W. B., Moore R. Y. Demonstration by immunoperoxidase histochemistry of calcitonin in the anterior lobe of the rat pituitary. J Clin Endocrinol Metab. 1978 Aug;47(2):457–460. doi: 10.1210/jcem-47-2-457. [DOI] [PubMed] [Google Scholar]
  11. Dietrich F. M., Hunziker W. H., Fischer J. A. Synthetic human calcitonin: analysis of antibodies obtained from various animal species and determination of immunoreactive hormone in human sera. Acta Endocrinol (Copenh) 1975 Nov;80(3):465–486. doi: 10.1530/acta.0.0800465. [DOI] [PubMed] [Google Scholar]
  12. Findlay D. M., Michelangeli V. P., Eisman J. A., Frampton R. J., Moseley J. M., MacIntyre I., Whitehead R., Martin T. J. Calcitonin and 1,25-dihydroxyvitamin D3 receptors in human breast cancer cell lines. Cancer Res. 1980 Dec;40(12):4764–4767. [PubMed] [Google Scholar]
  13. Findlay D. M., deLuise M., Michelangeli V. P., Ellison M., Martin T. J. Properties of a calcitonin receptor and adenylate cyclase in BEN cells, a human cancer cell line. Cancer Res. 1980 Apr;40(4):1311–1317. [PubMed] [Google Scholar]
  14. Fischer J. A., Sagar S. M., Martin J. B. Characterization and regional distribution of calcitonin binding sites in the rat brain. Life Sci. 1981 Aug 17;29(7):663–671. doi: 10.1016/0024-3205(81)90018-7. [DOI] [PubMed] [Google Scholar]
  15. Freed W. J., Perlow M. J., Wyatt R. J. Calcitonin: inhibitory effect on eating in rats. Science. 1979 Nov 16;206(4420):850–852. doi: 10.1126/science.493987. [DOI] [PubMed] [Google Scholar]
  16. Fritsch H. A., Van Noorden S., Pearse A. G. Localization of somatostatin-, substance P- and calcitonin-like immunoreactivity in the neural ganglion of Ciona intestinalis L. (Ascidiaceae). Cell Tissue Res. 1979 Nov;202(2):263–274. doi: 10.1007/BF00232240. [DOI] [PubMed] [Google Scholar]
  17. Galan Galan F., Rogers R. M., Girgis S. I., Arnett T. R., Ravazzola M., Orci L., MacIntyre I. Immunochemical characterization and distribution of calcitonin in the lizard. Acta Endocrinol (Copenh) 1981 Jul;97(3):427–432. doi: 10.1530/acta.0.0970427. [DOI] [PubMed] [Google Scholar]
  18. Galan Galan F., Rogers R. M., Girgis S. I., MacIntyre I. Immunoreactive calcitonin in the central nervous system of the pigeon. Brain Res. 1981 May 11;212(1):59–66. doi: 10.1016/0006-8993(81)90032-9. [DOI] [PubMed] [Google Scholar]
  19. Girgis S. I., Galan F. G., Arnett T. R., Rogers R. M., Bone Q., Ravazzola M., MacIntyre I. Immunoreactive human calcitonin-like molecule in the nervous systems of protochordates and a cyclostome, Myxine. J Endocrinol. 1980 Dec;87(3):375–382. doi: 10.1677/joe.0.0870375. [DOI] [PubMed] [Google Scholar]
  20. Heath H., 3rd, Edis A. J. Pheochromocytoma associated with hypercalcemia and ectopic secretion of calcitonin. Ann Intern Med. 1979 Aug;91(2):208–210. doi: 10.7326/0003-4819-91-2-208. [DOI] [PubMed] [Google Scholar]
  21. Iwasaki Y., Chihara K., Iwasaki J., Abe H., Fujita T. Effect of calcitonin on prolactin release in rats. Life Sci. 1979 Oct 1;25(14):1243–1248. doi: 10.1016/0024-3205(79)90467-3. [DOI] [PubMed] [Google Scholar]
  22. Kaplan E. L., Peskin G. W., Arnaud C. D. Nonsteroid, calcitonin-like factor from the adrenal gland. Surgery. 1969 Jul;66(1):167–174. [PubMed] [Google Scholar]
  23. Loreau N., Lajotte C., Wahbe F., Ardaillou R. Effects of guanyl nucleotides on calcitonin-sensitive adenylate cyclase and calcitonin binding in rat renal cortex. J Endocrinol. 1978 Mar;76(3):533–545. doi: 10.1677/joe.0.0760533. [DOI] [PubMed] [Google Scholar]
  24. Margules D. L., Flynn J. J., Walker J., Cooper C. W. Elevation of calcitonin immunoreactivity in the pituitary and thyroid glands of genetically obese rats (fa/fa). Brain Res Bull. 1979 Sep-Oct;4(5):589–591. doi: 10.1016/0361-9230(79)90098-4. [DOI] [PubMed] [Google Scholar]
  25. Moran J., Hunziker W., Fischer J. A. Calcitonin and calcium ionophores: cyclic AMP responses in cells of a human lymphoid line. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3984–3988. doi: 10.1073/pnas.75.8.3984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Nakamuta H., Furukawa S., Koida M., Yajima H., Orlowski R. C., Schlueter R. Specific binding of 125I-salmon calcitonin to rat brain: regional variation and calcitonin specificity. Jpn J Pharmacol. 1981 Feb;31(1):53–60. doi: 10.1254/jjp.31.53. [DOI] [PubMed] [Google Scholar]
  27. Nakanishi S., Inoue A., Kita T., Nakamura M., Chang A. C., Cohen S. N., Numa S. Nucleotide sequence of cloned cDNA for bovine corticotropin-beta-lipotropin precursor. Nature. 1979 Mar 29;278(5703):423–427. doi: 10.1038/278423a0. [DOI] [PubMed] [Google Scholar]
  28. Neher R., Riniker B., Maier R., Byfield P. G., Gudmundsson T. V., MacIntyre I. Human calcitonin. Nature. 1968 Dec 7;220(5171):984–986. doi: 10.1038/220984a0. [DOI] [PubMed] [Google Scholar]
  29. Pavlinac D. M., Lenhard L. W., Parthemore J. G., Deftos L. J. Immunoreactive calcitonin in human cerebrospinal fluid. J Clin Endocrinol Metab. 1980 Apr;50(4):717–720. doi: 10.1210/jcem-50-4-717. [DOI] [PubMed] [Google Scholar]
  30. Pearse A. G., Takor T. Embryology of the diffuse neuroendocrine system and its relationship to the common peptides. Fed Proc. 1979 Aug;38(9):2288–2294. [PubMed] [Google Scholar]
  31. Pecile A., Ferri S., Braga P. C., Olgiati V. R. Effects of intracerebroventricular calcitonin in the conscious rabbit. Experientia. 1975 Mar 15;31(3):332–333. doi: 10.1007/BF01922569. [DOI] [PubMed] [Google Scholar]
  32. Rizzo A. J., Goltzman D. Calcitonin receptors in the central nervous system of the rat. Endocrinology. 1981 May;108(5):1672–1677. doi: 10.1210/endo-108-5-1672. [DOI] [PubMed] [Google Scholar]
  33. Voelkel E. F., Tashjian A. H., Jr, Davidoff F. F., Cohen R. B., Perlia C. P., Wurtman R. J. Concentrations of calcitonin and catecholamines in pheochromocytomas, a mucosal neuroma and medullary thyroid carcinoma. J Clin Endocrinol Metab. 1973 Aug;37(2):297–307. doi: 10.1210/jcem-37-2-297. [DOI] [PubMed] [Google Scholar]
  34. Wieland P., Fischer J. A., Trechsel U., Roth H. R., Vetter K., Schneider H., Huch A. Perinatal parathyroid hormone, vitamin D metabolites, and calcitonin in man. Am J Physiol. 1980 Nov;239(5):E385–E390. doi: 10.1152/ajpendo.1980.239.5.E385. [DOI] [PubMed] [Google Scholar]
  35. Yamamoto I., Morita R., Fukunaga M., Dokoh S., Shigeno C., Torizuka K., Noda T. Adenylate cyclase activation and competitive binding with renal tissue using synthetic eel calcitonin analog and its fragments. Endocrinology. 1981 Feb;108(2):698–702. doi: 10.1210/endo-108-2-698. [DOI] [PubMed] [Google Scholar]
  36. Yamamoto M., Tachikawa S., Maeno H. Evoked potential studies of porcine calcitonin in rabbits. Neuropharmacology. 1981 Jan;20(1):83–86. doi: 10.1016/0028-3908(81)90047-2. [DOI] [PubMed] [Google Scholar]
  37. Zileli M. S., Güner T., Adalar N. Evidence for a hypocalcemic factor in the hypothalamus. Experientia. 1972 Feb 15;28(2):204–205. doi: 10.1007/BF01935761. [DOI] [PubMed] [Google Scholar]

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