Figure 3. siRNA-induced knock down of EGFR evaluated by IC-NIR imaging.

HT-29 cells were transfected for 2 days with 5 nM anti-EGFR Silencer select siRNA or scrambled RNA or left untreated (control). Evaluation of EGFR expression was performed by In Cell NIR imaging using 7 nM EGF-NIR (black bars) and western blotting (gray bars). The values are mean ± SD (n = 3). * p<0.05 vs. scrambled or control.