Figure 3. DRBD3 does not associate with polysomes.

Trypanosome lysates were subjected to sucrose gradient separation in the absence or presence of translational inhibitors. Absorption at 254 nm was recorded during fractionation. Fractions were analyzed by western blot using an anti-DRBD3 antiserum. The distribution of DRBD3 along the gradients was compared to those of proteins known to be polysome-associated (ribosomal protein P0 and PABP2) or not bound to polysomes (BiP).