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. 2012 Nov 8;7(11):e48870. doi: 10.1371/journal.pone.0048870

Figure 4. Arsenite- and starvation-induced stress affects DRBD3 complex composition.

Figure 4

DRBD3 protein complexes were TAP-purified from cells incubated with 50 µM sodium arsenite for 3 h (A) or starved in PBS for 3 h (B), and compared to TAPs carried out with lysates obtained from equivalent amounts of normally grown trypanosomes (control). (C) Cell lines were generated that expressed both TAP-DRBD3 and 4xTy-tagged versions of PABP1 or PABP2. The presence of PABPs was assayed in the presence or absence of arsenite by western blot after IgG chromatography and TEV cleavage. (D) Association of Ty-PABP1, Ty-PABP2 and Ty-HSP70 with DRBD3 in starvation conditions was also analyzed as in panel C. (E) We also generated cell lines expressing TAP- or PTP-tagged versions of PABP1, PABP2 and HSP70. The amount of DRBD3 that copurified with these proteins in starvation conditions was analyzed after IgG chromatography and TEV cleavage using a DRBD3 antiserum.