Figure 2. R. toruloides fatty acid synthase system.

(a) Schematic representation of the transcriptional map and domain architecture of FAS. The FAS1 and FAS2 genes localized to the same scaffold and were separated by approximately 250 kb, and the entire transcripts were reconstructed from RNA-seq data. TAR, transcription active region; Transcript, reconstructed transcript; CDS, coding sequence; Ex lev, expression level, log₂(RNA-seq reads coverage); AT, acyltransferase; ER, enoyl reductase; DH, dehydratase; MPT, malonyl/palmitoyl transferase; ACP, acyl carrier protein; KR, ketoacyl reductase; KS, ketoacyl synthase; PPT, phosphopantetheinyl transferase. Asterisks represent the conserved serine residues for 4′-phosphopantetheine attachment. (b) Unique peptide coverage that was identified by LC-MS/MS.