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. 2012 Oct 16;3:1136. doi: 10.1038/ncomms2136

Figure 4. Relationship between SVH-2 receptor tyrosine kinase and MLK-1 in axon regeneration.

Figure 4

(a) Percentages of axons that initiated regeneration 24 h after laser surgery. Young adult animals were treated with ethanol or AEA for 6 h after axotomy. At least 30 axons in each condition were assayed. Error bars indicate 95% confidence interval (CI). ***P<0.001 as determined by Fisher's exact test. NS, not significant. (b) Effect of SVH-2 on the interaction between MLK-1 and SHC-1. Schematic diagrams of SVH-2 and Tpr::SVH-2C are shown in the upper part. COS-7 cells were transfected with plasmids encoding FLAG-Tpr::SVH-2C (WT), FLAG-Tpr::SVH-2C(K767R) (KR), HA-MLK-1 and T7-SHC-1 as indicated. Whole-cell extracts and immunoprecipitated complexes obtained with anti-HA (IP: HA) or anti-T7 antibody (IP: T7) were analysed by immunoblotting. pY: anti-phospho-tyrosine antibody. LZ: leucine zipper. (c) Schematic model for regulation of the KGB-1 JNK pathway by AEA-GOA-1/EGL-30-TPA-1 and SVH-1-SVH-2 in axon regeneration.