Fig. 3.

a Fraction of cells (%) expressing CD83, CD14 and HLA-DR by FACS analysis on day 8. After differentiation of day 6 iDC, cells were either kept in cultures under constant conditions for another 48 h (IPA-iDC, CRF-iDC, NC-iDC) or were supplemented with poly(I/C) for 48 h (IPA-mDC, CRF-mDC, NC-mDC). n ≥ 3 for NC-DC (controls). Significant differences were observed between iDC and mDC regarding CD83 (** p < 0.01) and HLA-DR-expression (* p = 0.01). No significant differences of CD14 expression in relation to the method of cryopreservation were noted (p = 0.35). b Histograms for CD83 staining of mature DC [positive fraction (%)], prepared from NC-PBMC, IPA-PBMC, and CRF-PBMC. One representative experiment of the series depicted in (a) is shown. Furthermore, mean fluorescence intensity of CD83 did not show significant differences between cryopreservation protocols