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. 2012 Aug 20;4(5):801–806. doi: 10.3892/etm.2012.676

Figure 2.

Figure 2

IDH2 expression levels in HCT-8 cells. (A) Expression of IDH2 mRNA was altered in HCT-8 cells transfected with IDH2 siRNA or the overexpression plasmid as detected by quantitative real-time RT-PCR. The experiment was performed in triplicate and repeated twice. Error bars represent the standard deviation, with *P<0.05 and **P<0.01 by the Student’s t-test. 1, HCT-8 cells transfected with PGPU6/GFP/Neo-IDH2 plasmid; 2, HCT-8 cells transfected with PGPU6/ GFP/Neo vector plasmid; 3, HCT-8 cells transfected with pEGFP-C1-IDH2 plasmid; 4, HCT-8 cells transfected with pEGFP-C1 vector plasmid; 5, HCT-8 cells without plasmid. (B) Western blot analysis confirmed a marked reduction in IDH2 protein levels in cells transfected with IDH2 siRNA and markedly increased expression in cells transfected with the IDH2 expression vector. The cells transfected with empty vector and the untreated cells were used as controls. (a) the IDH2 group; (b) the β-actin group.