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. 2012 Aug 20;4(5):801–806. doi: 10.3892/etm.2012.676

Figure 3.

Figure 3

The activity of isocitrate dehydrogenase (IDH) in HCT-8 cells. The IDH activity in cells transfected with IDH-siRNA was lower than that in the control cells. The cells transfected with the empty vector and untreated cells were used as controls. The experiment was performed in triplicate and repeated twice. Error bars represent the standard deviation, and *P<0.05 by a Student’s t-test. 1, HCT-8 cells transfected with the PGPU6/GFP/Neo-IDH2 plasmid; 2, HCT-8 cells transfected with the PGPU6/GFP/Neo vector plasmid; 3, HCT-8 cells transfected with the pEGFP-C1-IDH2 plasmid; 4, HCT-8 cells transfected with the pEGFP-C1 vector plasmid; 5, HCT-8 cells without plasmid.