Figure 4.

The growth of HCT-8 cell is affected by the expression of IDH2. An MTT assay was used to assess HCT-8 cell growth after reduction or overexpression of IDH2. (A) Cell growth was significantly decreased by transient transfection of PGPU6/GFP/Neo-IDH2 starting on day 3 post-transfection compared to controls. The cells transfected with the PGPU6/GFP/Neo empty vector and untreated cells were used as controls. (B) Cell growth increased after transient transfection of the pEGFP-C1-IDH2 vector by Day 3 post-transfection compared to controls. The cells transfected with pEGFP-C1 empty vector and untreated cells were used as controls. The experiment was performed in triplicate and repeated twice. Error bars represent the standard deviation, and ^*P<0.05 and ^**P<0.01 was statistically significant as determined by the Student’s t-test.