Table I.
Novel candidate microRNAs in Klinefelter syndrome and healthy control libraries.
| Name | Sequence | Arm | Length | Location | Precursor length (nt) | MFE | Count |
|---|---|---|---|---|---|---|---|
| miRNA-01 | AAAGGGGACAGCTCACAGGATT | 5′ | 22 | chr4:16229484:16229558:+ | 75 | −22.2 | 9 |
| miRNA-02 | AAATGAATCATGTTGGGCCTGT | 3′ | 22 | chr10:115051380:115051452:- | 73 | −53.8 | 10 |
| miRNA-03 | AACAAGAGAGCAGAACGAGGTT | 5′ | 22 | chr6:6152172:6152250: − | 79 | −22.5 | 32 |
| miRNA-04 | AAGGTAGCGGGAGGGTTGGGCT | 3′ | 22 | chr3:45883723:45883820:+ | 98 | −40.5 | 8 |
| miRNA-05 | ACTGGCAAAAGGGTTTAGAACT | 5′ | 22 | chr16:50830166:50830246:+ | 81 | −20.34 | 30 |
| miRNA-06 | AGAGGAGGGTGGAGCAAGTGGT | 5′ | 22 | chr2:68622734:68622824:+ | 91 | −31.8 | 40 |
| miRNA-07 | AGCAGAGACGTTGGAACTGGGCT | 5′ | 23 | chr1:150534435:150534511: − | 77 | −28.6 | 8 |
| miRNA-08 | CATGGCACTGGAGTAGAGCAT | 3′ | 21 | chr8:141181858:141181951: − | 94 | −33.5 | 14 |
| miRNA-09 | CCCTGGGGTTCTGAGGACATG | 5′ | 21 | chr9:35710651:35710743: − | 93 | −39 | 711 |
| miRNA-10 | GAGAGCTCCGACTGCAGCTGC | 3′ | 21 | chr11:133027217:133027311: − | 95 | −35.3 | 20 |
| miRNA-11 | GAGGACCCTGCAGGAATGGACG | 5′ | 22 | chr11:122650500:122650575:+ | 76 | −33.7 | 10 |
| miRNA-12 | GATGAGGAGGATGAGGAGGATG | 5′ | 22 | chr6:3616133:3616211:+ | 79 | −21.7 | 14 |
| miRNA-13 | GGAGGAACCTTGGAGCTTCGGCA | 3′ | 23 | chr22:31556037:31556127: − | 91 | −45.3 | 70 |
| miRNA-14 | TAAGCATGCTGTCTTTCTGGAG | 3′ | 22 | chr15:28518177:28518257: − | 81 | −20.8 | 7 |
| miRNA-15 | TAGGCCATTTTGGAAGCTGTTT | 5′ | 22 | chr2:33498389:33498471:+ | 83 | −23 | 19 |
| miRNA-16 | TCAGGCCAGGCTGGGAGGATGC | 5′ | 22 | chr1:55784355:55784424:+ | 70 | −52.4 | 8 |
| miRNA-17 | TCCTGGAGCTGGGCAGATGGGA | 5′ | 22 | chr19:2762628:2762702: − | 75 | −35.7 | 5 |
| miRNA-18 | TCCTGTACTGAGCTGCCCCGAGC | 5′ | 23 | chr8:41517952:41518035:+ | 84 | −59.1 | 18 |
| miRNA-19 | TCGACTTGCTCGGGCCCGGCT | 3′ | 21 | chr10:104402512:104402584: − | 73 | −34 | 15 |
| miRNA-20 | TCGGGCGGGAGTGGTGGCTTTT | 3′ | 22 | chr6:28918819:28918903:+ | 85 | −22.2 | 233 |
| miRNA-21 | TGGGCAGGGGCTTATTGTAGGAGT | 5′ | 24 | chr6:32137807:32137893: − | 87 | −30.1 | 97 |
| miRNA-22 | TGGGGCTGCGGTGGGTCGGGA | 3′ | 21 | chr21:45209209:45209298: − | 90 | −46.6 | 27 |
| miRNA-23 | TTGAGGGGAGAATGAGGTGGAGA | 5′ | 23 | chr1:43830309:43830391: − | 83 | −33.5 | 11 |
| miRNA-24 | TTGCTGGGAAAGGGAGAAGTTCAT | 3′ | 24 | chr8:1703286:1703370: − | 85 | −46.8 | 8 |
| miRNA-25 | TTGGAGGGTGTGGAAGACAT | 5′ | 20 | chr19:13051290:13051374:+ | 85 | −31 | 10 |
Name, the name of novel microRNAs in Klinefelter syndrome and healthy control libraries; sequence, microRNA sequence cloned in the small RNA library; arm, the microRNA location in the predicted hairpin structure 5′ or 3′ arm; length, the length of microRNA (bp); location, microRNA location in the chromosome; precursor length, the length of precursor microRNA; MFE, minimum free energy; count, the counts of microRNA reads.