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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: J Neurochem. 2012 Oct 11;123(5):689–699. doi: 10.1111/j.1471-4159.2012.07918.x

Fig. 1.

Fig. 1

Characterization of raft and non-raft domains isolated from SPMs of 6-mos F344/BNF1 rat brains. (A) Analysis of cholesterol and protein content of the 10 fractions collected from the sucrose density gradient as described under Methods. Data are means ± SEM obtained from 6 animals. (B) Cholera toxin and immunoblot detection of raft markers and PMCA. Eight μg of protein from fractions 2–9 were loaded on the gel for GM1, FLT-1, and pan PMCA detection, and 15 μg of protein for Thy-1 detection. The GM1 was detected with cholera toxin as described in the Methods. The dilutions for the various antibodies were: FLT-1 (1:250), Thy-1 (1:250), and pan PMCA (1:1000). Representative blots from 3 independent experiments with similar results are shown.