Skip to main content
. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: J Neurochem. 2012 Oct 11;123(5):689–699. doi: 10.1111/j.1471-4159.2012.07918.x

Fig. 5.

Fig. 5

Effects of aging on cholesterol to protein ratios and on PMCA levels in rafts and non-raft fractions. (A) Rafts (fractions #2–4) were collected from animals at 6, 23, and 34 mos. The total cholesterol was determined with Amplex Red and the bicinchoninic assay was used to determine the total protein in each sample. The cholesterol to protein ratio for the rafts at 6 mos was set to 1.0, and the ratios for the 23 and 34 mos samples calculated relative to the 6 mos value. Data represent the means ± SEM of the ratios from 5 sets of animals, and the significance of differences between samples were assessed by Student’s t test. * = p< 0.02. (B) Immunoblot analysis of PMCA levels was carried out with the pan PMCA antibody on pooled fractions #2–4, #5–6, and #7–9, prepared from the SPMs of 6, 23, and 34 mos F344BNF1 rat brains. (C) Relative immunoreactivities with the pan PMCA antibody were quantified in the fractions from 5 sets of animals, and the means ± SEM are shown. One-way Anova analysis revealed a statistically significant age effect (p<0.03) on PMCA levels in the raft fraction (#2–4) though the levels of the protein are higher than in the non – raft fractions. PMCA levels in the 2 non-raft fractions showed no significant change with age.