Figure 1.

Mutations in MED12 exon 2. The whole MED12 exon 2 with the amino acids and codon numbers is shown at the top, and multispecies alignment of the regions with the detected mutations is shown below. Mutations observed in uterine leiomyosarcoma and CRC samples are marked with black and red, respectively. Mutations G44C and G44V in ULMS were reported by Pérot et al (2012). Mutation G44C in CRC was observed in this study and also in the study by The Cancer Genome Atlas Network (2012). Mutation D34Y in CRC has been reported by Je et al (2012). Amino acids at the top are color-coded according to their side-chains' pK_as (acid dissociation constant) and charge at physiological pH 7.4. Red = negatively charged, green = hydrophobic, yellow = small non-polar, magenta = polar, blue = positively charged.