Figure 5.
Investigation of the mechanism of the synergy between CYC3 and paclitaxel. (A) 0.5 × 106 PANC-1 cells were seeded in 6-cm dishes with 3 ml DMEM media for 24 h before being treated with 3 nℳ paclitaxel±1 μℳ CYC3 for 3 h before collecting. The amount of paclitaxel in cells and media were measured by LC-MS. (B) Flow cytometry analysis of the cell cycle profiles of cells upon treatments as indicated. (C, D) MIA PaCa-2 (C) and PANC-1 (D) cells were treated as indicated for 24 and 48 h before collecting. The protein expression levels were determined by western blot. (E, F) MIA PaCa-2 (E) and PANC-1 (F) cells were treated as indicated for 24, 48 and 72 h. The secreted caspases-cleaved keratin 18 in the media was measured by M30 Apoptosense ELISA (Enzo Life Sciences).