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. 2012 Sep 17;287(46):38408–38425. doi: 10.1074/jbc.M112.415505

FIGURE 2.

FIGURE 2.

Mossy fiber infrapyramidal bundle is significantly shorter in BACE1−/− than wild-type mice. Coronal sections (40 μm) from brains of 3-month-old BACE1+/+ and BACE1−/− mice were immunostained for SPO (green; A and C) or BACE1 (red; B and D) and imaged with confocal microscopy. Representative sections of BACE1+/+ (A and B) and BACE1−/− (C and D) are shown. Note that the length of the BACE1−/− IPB is markedly shorter than that of the BACE1+/+ IPB (distances between the red arrows). Scale bar in D, 250 μm for A and D. E, lengths of the IPBs were determined using a free-form line measurement in ImageJ beginning at the point where the pyramidal cell layer ends and ending with the most distal point of continuous synaptoporin labeling inferior to the pyramidal cell layer. IPB lengths were normalized using a measurement of the distance from the beginning of the suprapyramidal bundle to the distal end of the slu, and the ratios were averaged across three sections per brain (E). The IPBs of BACE1−/− mice were significantly shorter than those of BACE1+/+ mice: 0.42 ± 0.020 versus 0.57 ± 0.011 normalized length (error bars, S.E.; n = 7; ***, p < 0.0001).