FIGURE 2.

FGF signaling regulates miR abundance through transcriptional and post-transcriptional mechanisms. A, simplified pathway of miR processing, showing the location of primers for detecting pri- or pre- miR transcripts. Pol II, RNA polymerase II. B, -fold change in abundance of pri-, pre-miRs, and mature miRs in control versus SU5402-treated embryos. Pri-miR levels are increased for all FGF-regulated miRs except miR-130b. Only miR-130b and -19b show increased pre-miRs levels, whereas miR-9 shows a decrease in pre-miRs. Error bars indicate S.E.