FIGURE 1.

Limited levels of Nup50 associate with Nup153. A, schematic depicting the three regions of Nup50, designated N, F, and R (9), as well as the sequence differences between the long and short isoforms of Nup50, as annotated previously (11). B, Nup153 was immunoprecipitated (I.P.) from lysates of HeLa cells expressing GFP or Nup50L (50L)-GFP (lanes 5 and 6); material isolated with equivalent levels of protein A-Sepharose beads alone was run alongside (lanes 3 and 4). The precipitated material was immunoblotted for the presence of Nup153, Nup50 (both recombinant and endogenous), and GFP. For comparison, samples of the input material are shown (7.5% input for Nup153 and Nup50 detection and 10% input for GFP detection) (lanes 1 and 2). The immunoglobulin heavy chain (HC) and light chain (LC) and an unknown background band (*) are indicated. Tfx, transfection.