FIGURE 2.

Nup153 interacts with the N-terminal domains of both Nup50 isoforms. A panel of Nup50-derived and control GFP fusion proteins were expressed in HeLa cells and recovered on an affinity matrix as indicated. Immunoblotting was performed to track the co-recovery of Nup153 (upper panels) along with the recovery of GFP fusion protein (middle left panel and lower right panel). The input levels of these proteins were assessed in parallel (5% loaded for Nup153 and 10% loaded for GFP) (lanes 1–7). The levels of α-tubulin (αTub) were probed (lower left panel) to confirm that equivalent levels of lysate were used for each condition. Molecular mass markers indicated are 130, 100, 70, 55, 40, and 35 kDa. S, short; L, long.