Skip to main content
. 2012 Sep 24;287(46):38515–38522. doi: 10.1074/jbc.M112.378893

FIGURE 3.

FIGURE 3.

Nup50 interacts with two distinct sites within Nup153. A, schematic of Nup153 with the unique N-terminal region (N), the zinc finger domain (Z), and the FG-rich C-terminal region (C) indicated. The gray box indicates an interface between Nup153 and Nup50 identified in Fig. 4. B, GFP proteins were recovered from lysates of cells expressing GFP alone or GFP fusions of the N-terminal, zinc finger, or C-terminal domain of Nup153. Recovery of Nup50 (upper panels) along with each GFP protein (lower panels) was tracked by immunoblotting. Molecular mass markers indicated are 130, 100, 70, 55, 40, and 35 kDa. C, lysates of cells expressing GFP fusions of Nup153-N, Nup153-Z, or Nup153-C in conjunction with either the HA-tagged Nup50S-N (S) or Nup50L-N (L) were used for a GFP-Trap assay. Immunoblotting was performed to track the co-recovery of the Nup50 fragments (anti-HA; upper panels) along with the recovery of GFP fusion protein (lower panels).