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. 2012 Sep 24;287(46):38531–38542. doi: 10.1074/jbc.M111.336925

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — CS abundance is regulated by HYAL1 expression levels. A and B, constitutive OE of HYAL1 in C2C12 cells abrogated the accumulation of CS on day 5 in DM culture (A), whereas its knockdown (KD) caused elevated CS levels on day 8 (B) (n = 6, parental cells; n = 3, OE#1; n = 4, OE#5; n = 6, KD#1; n = 6, KD#8; at each time point, results are expressed as means ± S.D.; *, p < 0.05; **, p < 0.005; ***, p < 0.001). C and D, HA contents were essentially unaltered in both HYAL1 OE (C) and KD (D) cells (n = 4, parental cells; n = 3, OE#1; n = 4, OE#5; n = 5, KD#1; n = 5, KD#8; at each time point, results are expressed as means ± S.D.).