FIGURE 3.

SALL4B is sumoylated on multiple sites. A, schematic presentations of wild-type SALL4B, as well as its various mutants. Lysine residues subjected to mutation into arginines are shown. HA and His₆ tags are fused in-frame at the C terminus. B, HEK293T cells were transfected with HA-tagged wild-type SALL4B (Wt) and various mutants with single or multiple lysine residues replaced with arginines for 48 h, after which equal amounts of cell lysates were blotted with antibodies to HA and β-actin. Blots of both short and long exposure are shown. C, schematic presentation of important residues of SALL4B for sumoylation and phosphorylation, as well as a histidine residue in the zinc finger domain mutation of which causes mild Okihiro syndrome.