Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Sep 25;287(46):38695–38704. doi: 10.1074/jbc.M112.395053

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 2. — Nonreducing SDS-PAGE analysis of unlocked and locked mutants. A, purified α₃β₃γ complexes were electrophoresed on 12% polyacrylamide gel. First lane, Cys-less; second and third lanes, unlocked and locked A28C/L267C; fourth and fifth lanes, unlocked and locked V32C/A268C; sixth and seventh lanes, unlocked and locked V32C/A264C; eighth and ninth, unlocked and locked A35C/A264C; 10th and 11th lanes, unlocked and locked V39C/A264C. B, the oxidation conditions of purified A28C/A268C, V32C/L267C, A35C/L267C, and A35C/A268C complexes were surveyed as described under “Experimental Procedures,” and the most representative results are shown. First and second lanes, unlocked and locked A28C/A268C; third and fourth lanes, unlocked and locked V32C/L267C; fifth and sixth lanes, unlocked and locked A35C/L267C; seventh and eighth lanes, unlocked and locked A35C/A268C. C, efficiency of disulfide bond formation was determined from the band intensities of unlocked and locked γ subunits shown in A and B. The combination which was not prepared in this study is shown by a x.