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Apparent dissociation constants for the ϵ subunit with lock mutants
Apparent dissociation constants (KD) for the ϵ subunit with unlocked and locked mutants were determined by averaging the results of three independent titration data with the hyperbolic equation as described under “Experimental Procedures.”
| Complex |
KD |
|
|---|---|---|
| Unlocked | Locked | |
| nm | ||
| Cys-less | 0.93 ± 0.29 | |
| V32C/A268C | 2.0 ± 0.6 | 1.6 ± 0.1 |
| V32C/A264C | 1.2 ± 0.2 | 2.2 ± 0.2 |
| A35C/A264C | 1.2 ± 0.2 | 1.4 ± 0.1 |
| V39C/A264C | 1.6 ± 0.5 | 2.4 ± 0.2 |
