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Synchronous fluorescence spectra of hAS3MT (Δλ = 60 nm) in different systems
| Parameter | Effect of AdoMet on rhAS3MT |
|||
|---|---|---|---|---|
| Phosphate buffer (25.00 mm) | GSH (7.00 mm) | Cysteine (10.00 mm) | TCEP (0.70 mm) | |
| F0 | 797.98 | 680.60 | 680.54 | 869.86 |
| F | 624.85 | 500.08 | 483.80 | 700.56 |
| λ0 (nm) | 277.0 | 279.0 | 279.5 | 278.0 |
| λ (nm) | 281.5 | 282.0 | 282.5 | 281.5 |
| (F0 − F)/F0 (%) | 21.70 | 26.52 | 28.91 | 19.46 |
| Δλ (nm) | 4.5 | 3.0 | 3.0 | 3.5 |
