FIGURE 5.

HPLC-ESI⁻-MS/MS analysis of hpol κ in vitro replication products on 1,N⁶-γ-HMHP-dA-adduct containing template. Primer-template complexes (100 pmol) were incubated at 37 °C for 4 h in the presence of hpol κ (40 pmol) and the four dNTPs. The primer extension products were subjected to UDG hydrolysis and piperidine treatment to yield shorter oligonucleotide fragments that can be sequenced by HPLC-ESI-MS/MS. A, Total Ion Chromatogram of primer extension mixtures; B, mass spectra of the primer extension products eluting between 10 and 13 min.