FIGURE 3.

Multiple alignment of PCu_AC amino acidic sequences. The secondary structure of T. thermophilus PCu_AC extracted from its crystal structure (Protein Data Bank code 2K6W) is shown above the alignment. The numbering refers to the T. thermophilus PCu_AC sequence. B. japonicum PcuC and Blr7088, R. sphaeroides 2.4.1 RSP_2017, Rhodopseudomonas palustris HaA2 RPB_2549, Methylobacterium extorquens PA1 Mext_1379, Pseudomonas fluorescens Pf0-1Pfl01_0598, Vibrio cholerae HC-02A1 VCHC02A1_2964, and Nitrobacter hamburgensis X14 Nham_2200 sequences are included in the alignment. Similarity and identity columns are shown as empty boxes and white on black type, respectively. Copper-binding residues of TtPCu_AC are marked with triangles; the corresponding residues of PcuC are His⁷⁹, Met⁹⁰, His¹¹³, and Met¹¹⁵. The gray-shaded amino acids at the C-terminal end of the PcuC sequence are those that were removed to test if they bind copper. a, the C-terminal 118-amino acid domain of Blr7088 was omitted in the alignment.