TABLE 2.
Symbiotic properties of ΔpcuABCDE mutants inoculated on soybean
Mutants were tested in parallel with the wild type in three independent experiments. Shown here are data from one representative experiment.
| Strain | Relevant genotypea | Number of nodules | Nodule dry weight | Nitrogenase activityb | Relative Fix activityc |
|---|---|---|---|---|---|
| mg | %/min/g | % | |||
| 110spc4 | Wild type | 20.4 ± 4.0 | 2.4 ± 0.7 | 3.0 ± 0.7 | 100 ± 22.3 |
| 6611 | ΔpcuABCDE-a | 25.8 ± 7.8 | 1.3 ± 0.3 | 0.8 ± 0.5 | 25.9 ± 16.9 |
| 6612 | ΔpcuABCDE-b | 23.3 ± 5.1 | 1.4 ± 0.4 | 0.7 ± 0.4 | 24.4 ± 12.5 |
a Strain 6611 has insert in the same orientation (-a) and strain 6612 in the opposite orientation (-b).
b Nitrogenase activity is expressed as percentage of C2H4 formed/min/g.
c Relative nitrogen fixation activity is expressed as a percentage of wild type.